Home
Contact
Sitemap
en English
Cart (empty) 0
Expert in organic products and insect control since 2002

Home

Brands

Same day dispatch

Express home delivery or relay point

Welcome offer from €75 on the site

New products

All new products

On sale

All sale products

Best sellers

All best sellers

Customer reviews

Study by the T.E.C laboratory of insecticide 4j

Laboratory tests of our insecticide 4J



Labo tests with insecticide 4J diluted to 10% - September 2016

Insecticide 4J - Testé par un laboratoire indépendant

Introduction and methodology


Insecticide 4JThe T.E.C. laboratory conducted tests to determine the biological effectiveness of the specialty " INSECTICIDE 4J 10% » intended for insect treatments. The trial consists of direct shooting of insects at a realistic dose and recording of mortality over time. The trial focuses on bed bugs, cat chips and tiger mosquitoes.

The treatments were made with sprayers for laboratory use. The amount of product actually applied was measured by weighing. The arthropods were held in a glass ring in the centre of the test room floor. At the same time, the ring was removed, the product was sprayed and a chronometer was activated.
The sprayer was 45 degrees and the beak was 30 cm away from the arthropods.
An experimenter announced time intervals and noted on a table the data by a second experimenter observing the decrease.
An arthropod was considered 'decrease' or 'death' if it could not move properly.

Then the dead arthropods were collected in Petri boxes for a 24-hour evaluation. The floor of the treatment area was washed and cleaned after the same procedure as the flying insect test (NF T 72-320).
Results Interpretation: Time to kill all insects (KT100) / 24-hour mortality rate of adults and nymphs.

3 rehearsals were performed.


Tests on the bed bug - Bed bug - Cimex lectularius


Installation of the bed bug test

la punaise de lit - Cimex lectularius

  1. Product tested
    The sample used for testing is as follows:
    INSECTICIDE 4J-10% - Manufacturing 26/08/2016
    The sample is kept 3 months in the laboratory for possible subsequent analyses.
  2. Dose to test
    We apply 3 ml /m2 of product 4J 10% on insects for direct firing tests.
  3. Target insects

    Bed bugs: Male and female adults + the nymph of a specialized institute (Pharm'Insect - France), raised under controlled conditions and fed with rabbit blood. Insects were used immediately. There was no insect acclimatization before the test. No anesthesia was used.

  • 3 lots (repetition) of 25 adults were exposed to the product.
  • 3 repetitions were conducted = 75 insects per factor.
  • Unprocessed lots are followed in parallel to verify the viability of living equipment.
Treatment roll-out
The solution is directly applied with a hand sprayer.
The experimenter recorded insect mortality at regular intervals, depending on the speed of action, to know the short-term kinetics of the effect. After a total exposure time of 1 hour, insects were transferred to inert surfaces not treated with nutritious substrate and water available. This was placed under climatic conditions. Experimental conditions

The observations made on arthropods must take into account these two types of effects:

Observation of the sacrificed effect (KD) : during the 1 hour exposure period, the effect was judged to be sacrificed by counting several times the number (number) of individuals moving normally and others. An observation was also made after 4:00.

Observation of the mortal effect: 24 hours after the insect exposure began, deaths and insects were counted. All immobile insects and without movement of their legs no longer reacting to mechanical stimulation were considered dead.


Results and conclusion

After a maximum time of 15 minutes, 100% of bed bugs were dead.

In the conditions of these tests, with the samples provided, insect strains and methods considered, speciality "INSECTICIDE 4J-concentration 10%" presented a very good insecticide and definitive efficiency vis-à-vis bed bugs.

The full TEC report and all results are available on the page: test-insecticide-4J



Cat chip trials - Cat flea - Ctenocephalides felis

Testing on the cat chip

la puce - Ctenocephalides felis

  1. Product tested
    The sample used for testing is as follows:
    INSECTICIDE 4J-10% - Manufacturing 26/08/2016
    The sample is kept 3 months in the laboratory for possible subsequent analyses.
  2. Dose to test
    We apply 3 ml /m2 of product 4J 10% on insects for direct firing tests.
  3. Target insects

    Cat bugs: Insects were raised by the University of Bristol (United Kingdom). Before trial, insects are raised under the following optimal conditions:

    - Temperature: 27°C +/ - 2°C
    - Relative humidity: 80 % +/ - 10 %,
    - Light: 16:8 hours (light 700lux: darkness) insects were used immediately. There was no acclimatization before the trial. No anesthesia was used.


    • 3 lots (repetition) of 25 adults were exposed to product + 24 nymphs
    • 3 repetitions were conducted = 75 insects per factor.
    • Unprocessed lots are followed in parallel to verify the viability of living equipment.
  4. Treatment roll-out
    The solution is directly applied with a hand sprayer.
    The experimenter recorded insect mortality at regular intervals, depending on the speed of action, to know the short-term kinetics of the effect. After a total exposure time of 1 hour, insects were transferred to inert surfaces not treated with nutritious substrate and water available. This was placed under climatic conditions.
  5. Experimental conditions

    The observations made on arthropods must take into account these two types of effects:

    Observation of the sacrificed effect (KD) : during the 1 hour exposure period, the effect was judged to be sacrificed by counting several times the number (number) of individuals moving normally and others. An observation was also made after 4:00.

    Observation of the mortal effect: 24 hours after the insect exposure began, deaths and insects were counted. All immobile insects and without movement of their legs no longer reacting to mechanical stimulation were considered dead.

Results and conclusion

After a maximum time of 15 minutes, 100% of the cat chips were dead.

In the conditions of these tests, with the samples provided, insect strains and methods considered, speciality "INSECTICIDE 4J-concentration 10%" has presented a very good insecticide and definitive efficiency vis-à-vis cat chips.

The full TEC report and all results are available on the page: test-insecticide-4J

Tiger mosquito tests - Aedes Albopictus



Testing on the Tiger mosquito

Le moustique tigre Aedes albopictus - Dengue, Chikungunya, Zika

  1. Product tested
    The sample used for testing is as follows:
    INSECTICIDE 4J-10% - Manufacturing 26/08/2016
    The sample is kept 3 months in the laboratory for possible subsequent analyses.
  2. Dose to test
    We apply 3 ml /m2 of product 4J 10% on insects for direct firing tests.
  3. Target insects

    Tiger mosquitoes: 50 +/-2 four to six days old females of Aedes albopictus, laboratory colony high since 1992 (insect origin: ORSTOM BONDY/QUI + SEAMAR).

    Settlement conditions: in a controlled climate room, kept at 28 +/-1°C, 75% +/-10 HR, and lit 700 lux 16 hours + darkness 8 hours.
    The mosquitoes are raised in the 50 cm compartment cages on the side and fed with water and sugar + blood flour for females.
    Females were starved with blood flour for 24 hours before testing. Insects were used immediately. There was no acclimatization before the trial. No anesthesia was used.


    • 3 lots (repetition) of 25 adults were exposed to the product
    • 3 repetitions were conducted = 75 insects per factor.
    • Unprocessed lots are followed in parallel to verify the viability of living equipment.
  4. Treatment roll-out
    The solution is directly applied with a hand sprayer.
    The experimenter recorded insect mortality at regular intervals, depending on the speed of action, to know the short-term kinetics of the effect. After a total exposure time of 1 hour, insects were transferred to inert surfaces not treated with nutritious substrate and water available. This was placed under climatic conditions.
  5. Experimental conditions

    The observations made on arthropods must take into account these two types of effects:

    Observation of the sacrificial effect (KD): during the 1 hour exposure period, the sacrificial effect was judged by counting several times the number (numer) of individuals moving normally and others. An observation was also made after 4:00.

    Observation of the mortal effect: 24 hours after the insect exposure began, deaths and insects were counted. All immobile insects and without movement of their legs no longer reacting to mechanical stimulation were considered dead.

Results and conclusion

After a maximum time of 10 minutes, 100% of the Tiger mosquitoes were dead.

In the conditions of these tests, with the samples provided, insect strains and methods considered, speciality "INSECTICIDE 4J-concentration 10%" presented a very good insecticide and definitive efficiency vis-à-vis Tiger mosquitoes.

The full TEC report and all results are available on the page: test-insecticide-4J